Project 170252

The different cell types within the human body all possess an identical DNA content. However, each cell-type has a specific subset of genes that it expresses which gives rise to its identity. Once a cell becomes committed to a cell lineage, a gene expression profile is established that is put into a memory that can then be passed on to daughter cells. This cellular memory is established through epigenetic modification of chromatin, and is mediated by the polycomb and trithorax groups of proteins. In this proposal, we plan to examine how specific cell types decide which genes are going to be marked by polycomb and trithorax group proteins. Using muscle cell development as a model system, we will examine the role of transcriptional regulators in targeting trithorax group proteins to muscle specific genes. Information gained through these studies will permit us to better understand how different cell types are established during development. This knowledge will allow us to take an informed approach to differentiating cells, and thus facilitate the development of efficient stem cell therapies.

chemical synthesis and modification of therapeutic oligonucleotides