Project 170406

The gastrointestinal (GI) tract and the brain are intimately connected and behavioral factors are important in the expression of common GI diseases such as Irritable Bowel Syndrome (IBS). IBS is the most common GI disorder in our society but its cause is poorly understood and treatment is largely symptom-based; it does not cure. This condition is frequently accompanied by depression or anxiety and is considered by some to psychosomatic. Recent observations show that acute gastroenteritis can trigger IBS and that this may result in changes in the bacterial population that resides in the gut - known as flora. In this proposal, we will investigate whether changes in gut flora contribute to the expression of IBS using a mouse model. We have already shown that perturbing gut flora produces changes bowel function. As psychiatric conditions often accompany IBS, we will investigate whether the gut flora influence the brain and change behavior. The proposal uses molecular tools to identify gut bacteria and experiments are undertaken in a unique germ free laboratory to minimize contamination. If we succeed in showing that gut flora influence both gut function and behavior, then it will be possible to develop a single treatment strategy to treat all aspects of this condition.

investigation of NLR-mediated cytokine release and cell death pathways