Project 170783

Functional Characterization of Bone Sialoprotein

170783

Functional Characterization of Bone Sialoprotein

$550,272
Project Information
Study Type: Other Mechanistic_Study
Therapeutic Area: Orthopedics
Research Theme: Biomedical
Disease Area: bone repair
Data Type: Canadian
Institution & Funding
Principal Investigator(s): Goldberg, Harvey A; Hunter, Graeme K
Institution: University of Western Ontario
CIHR Institute: Musculoskeletal Health and Arthritis
Program: Operating Grant
Peer Review Committee: Clinical Investigation - B: Arthritis, Bone, Skin and Cartilage
Competition Year: 2008
Term: 4 yrs 0 mth
Abstract Summary

The organic matrices of mineralized tissues consist principally of type I collagen and a variety of non-collagenous proteins. These proteins are postulated to have critical roles in tissue development and the mineralization process. Studies conducted in our laboratories and elsewhere have shown that one of these proteins, bone sialoprotein, is a multifunctional protein that can interact with the mineral, cells and collagen. Bone sialoprotein can initiate mineral formation, promote osteoblast differentiation and thus new bone formation, in addition to activating osteoclasts, cells that resorb mineralized tissues. Using a wide spectrum of physical, chemical, biochemical and molecular techniques this study will focus on bone sialoprotein-collagen-mineral interactions. These studies will provide important information on the fundamental mechanism of mineralization in bone, and may lead to the development of novel therapeutic agents for the promotion of bone repair.

Research Characteristics

This project includes the following research characteristics:

Regulatory Pathway
Knowledge Translation Focus
Biomarker Endpoints
Study Justification

"this study will focus on bone sialoprotein-collagen-mineral interactions using a wide spectrum of physical, chemical, biochemical and molecular techniques"

Novelty Statement

"These studies will provide important information on the fundamental mechanism of mineralization in bone, and may lead to the development of novel therapeutic agents for the promotion of bone repair."

Methodology Innovation

using a wide spectrum of physical, chemical, biochemical and molecular techniques to study bone sialoprotein interactions

Keywords
Biomineralization Bone Formation And Repair Collagen Binding Hydroxyapatite Nucleation Osteoblast Differentiation Physical Chemical Characterization Of Hydroxyapatite Protein Structure And Function