Project 171062

Mechanisms of local and retrograde signaling by NGF in compartmented cultures of rat sympathetic neurons

171062

Mechanisms of local and retrograde signaling by NGF in compartmented cultures of rat sympathetic neurons

$430,946
Project Information
Study Type: Other Mechanistic_Study
Therapeutic Area: Neurology
Research Theme: Biomedical
Disease Area: neurodegenerative diseases, spinal cord injury, brain injury
Data Type: Canadian
Institution & Funding
Principal Investigator(s): Campenot, Robert B
Institution: University of Alberta
CIHR Institute: Neurosciences, Mental Health and Addiction
Program: Operating Grant
Peer Review Committee: Molecular & Cellular Neurosciences - B
Competition Year: 2008
Term: 3 yrs 0 mth
Abstract Summary

During the development of the nervous system 50% of the neurons that are generated die because their nerve fibers have failed to make correct connections that supply nerve growth factor (NGF) or a similar growth factor to the neurons. We have found that sympathetic neurons in specialized compartmented cultures die when their nerve endings are deprived of NGF because the removal of NGF generates a death signal in the nerve fibers that travels back to the cell body of the neuron and activates mechanisms that kill the neuron. We propose to investigate the detailed mechanism that generates the death signal and transports it to the cell body which will provide insight into how neurons can be kept alive in patients with neurodegenerative diseases or spinal cord or brain injury.

Research Characteristics

This project includes the following research characteristics:

Knowledge Translation Focus
Biomarker Endpoints
Study Justification

"We propose to investigate the detailed mechanism that generates the death signal and transports it to the cell body which will provide insight into how neurons can be kept alive in patients with neurodegenerative diseases or spinal cord or brain injury."

Novelty Statement

"We propose to investigate the detailed mechanism that generates the death signal and transports it to the cell body which will provide insight into how neurons can be kept alive in patients with neurodegenerative diseases or spinal cord or brain injury."

Methodology Innovation

using compartmented cultures of rat sympathetic neurons to study retrograde death signaling

Keywords
Compartmented Cultures Endocytosis Intracellular Signaling Nerve Growth Factor Neurodegenerative Disorders Neurotrauma