Project 171256
Importance of PGE2 in CCR7-dependent migration of monocytes/macrophages to lymph nodes and implication in HIV-1 dissemination.
Importance of PGE2 in CCR7-dependent migration of monocytes/macrophages to lymph nodes and implication in HIV-1 dissemination.
Project Information
| Study Type: | Other Mechanistic_Study |
| Therapeutic Area: | Hiv_Aids |
| Research Theme: | Biomedical |
| Disease Area: | HIV-1 |
| Data Type: | Canadian |
Institution & Funding
| Principal Investigator(s): | Dumais, Nancy |
| Institution: | Université de Sherbrooke |
| CIHR Institute: | Infection and Immunity |
| Program: | |
| Peer Review Committee: | Virology & Viral Pathogenesis |
| Competition Year: | 2008 |
| Term: | 3 yrs 0 mth |
Abstract Summary
After initial entry of the human immunodeficiency virus (HIV), replication of the virus first occurs at the site of infection. Then the major site of replication quickly shifts to lymphoid tissues of the body, including those in lymph nodes, spleen, liver, and bone marrow. Since macrophages are important both as reservoirs and vectors for the spread of HIV in the body, the main objective of the current proposal is to determine the cell surface molecule responsible for the migration of infected monocytes/macrophages. Our preliminary results indicate that expression and functionality of CCR7, a chemokine receptor, are up-regulated in monocytes in the presence of PGE2, a pro-inflammatory molecule. This research project is designed to gain novel insights on CCR7 at the molecular, cellular and animal levels. In the first objective, we will characterize CCR7 expression by PGE2 in macrophages. In vivo and in vitro models will be used to determine the impact of HIV-1 infection on CCR7 expression in monocytes and in macrophages. The data resulting from our investigations will serve to develop strategies to stop HIV dissemination.
Research Characteristics
This project includes the following research characteristics:
Study Justification
"This research project is designed to gain novel insights on CCR7 at the molecular, cellular and animal levels."
Novelty Statement
"The data resulting from our investigations will serve to develop strategies to stop HIV dissemination."
Methodology Innovation
investigating the role of PGE2 in up-regulating CCR7 expression on monocytes/macrophages and its implication for HIV-1 dissemination