Project 458127

Pairing pooled CRISPR/Cas9 screens with high-resolution multiplexed approaches to study cell competition and pre-malignancy in breast and skin epithelium

458127

Pairing pooled CRISPR/Cas9 screens with high-resolution multiplexed approaches to study cell competition and pre-malignancy in breast and skin epithelium

$105,000
Project Information
Study Type: Unclear
Research Theme: Biomedical
Institution & Funding
Principal Investigator(s): Teng, Katie
Supervisor(s): Schramek, Daniel; Jackson, Hartland W
Institution: Sinai Health System (Toronto)
CIHR Institute: Cancer Research
Program: Doctoral Research Award: Canada Graduate Scholarships
Peer Review Committee: Doctoral Research Awards - A
Competition Year: 2021
Term: 3 yrs 0 mth
Abstract Summary

Many healthy adult tissues, such as the breast and skin, contain cells that carry mutations that accumulate with age and are linked to cancer development. Although many cells are known to be mutated in normal tissues, mechanisms such as cell competition are in place to eliminate abnormal cells and maintain tissue health. However, certain alterations may give a cell a growth and/or proliferative advantage over healthy cells. These cell populations can then further evolve into precancerous lesions and eventually malignant tumours. It is, therefore, necessary to establish innovative systems to study the burden of mutations in phenotypically normal breast and skin tissues. From our study, we hope to unveil the earliest essential steps in breast and skin cancer development and unravel the molecular pathways implicated which will help guide the development of preventative, diagnostic and treatment strategies.

No special research characteristics identified

This project does not include any of the advanced research characteristics tracked in our database.

Keywords
Breast Cancer Cell Competition Crispr/Cas9 Functional Genomics Genetics Imaging Mass Cytometry Pre-Malignancy Single-Cell Spatial Phenotyping Skin Cancer Tumour Immune Microenvironment